zif268 (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc zif268

GPR81 mediates the regulation of HIIT on the AC-RAP1 and PLC-PKC pathways and ERK1/2 activation in the hippocampus of mice, as well as the expression of synaptic plasticity-related proteins. (A,D,H,M) Representative Western blotting images. (B,C,E,F) Quantitative analysis of AC, RAP1, PLC, and PKC protein expression levels in the hippocampus of different groups. (I–L) Quantitative analysis of protein expression levels of phosphorylated/total c-Raf and ERK1/2 in the hippocampus of different groups. (G) Quantitative analysis of gene expression levels of ERK1/2 in the hippocampus of each group. * p < 0.05, ** p < 0.01 vs . the SC group. ns, no significant difference. (N–S) Quantitative analysis of protein expression levels of NMDAR, PSD95, SYN, BDNF, c-FOS, and <t>Zif268</t> in the hippocampus of mice from different groups. Protein expression levels were normalized to Tubulin. Phosphorylated proteins were further normalized to their corresponding total protein levels. Relative gene expression was quantified and normalized to Gapdh. For comparisons among multiple groups, one-way ANOVA was applied. The data are presented as mean ± SD. n = 6. * p < 0.05, ** p < 0.01 vs . the SC group. ns, no significant difference. SC, scramble control, sedentary; SCH, scramble control + HIIT; GKD, GPR81 knockdown, sedentary; GKDH, GPR81 knockdown + HIIT.

Zif268, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 151 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/zif268/EGR1+Rabbit+mAb/pmc12846946-132-36-38
Average 95 stars, based on 151 article reviews

zif268 - by Bioz Stars, 2026-10

95/100 stars

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1) Product Images from "HIIT-induced lactate/GPR81 signaling with dual branches converging on ERK1/2 contributes to hippocampal synaptic remodeling and memory improvement"

Article Title: HIIT-induced lactate/GPR81 signaling with dual branches converging on ERK1/2 contributes to hippocampal synaptic remodeling and memory improvement

Journal: Frontiers in Cell and Developmental Biology

doi: 10.3389/fcell.2026.1699042

Figure Legend Snippet: GPR81 mediates the regulation of HIIT on the AC-RAP1 and PLC-PKC pathways and ERK1/2 activation in the hippocampus of mice, as well as the expression of synaptic plasticity-related proteins. (A,D,H,M) Representative Western blotting images. (B,C,E,F) Quantitative analysis of AC, RAP1, PLC, and PKC protein expression levels in the hippocampus of different groups. (I–L) Quantitative analysis of protein expression levels of phosphorylated/total c-Raf and ERK1/2 in the hippocampus of different groups. (G) Quantitative analysis of gene expression levels of ERK1/2 in the hippocampus of each group. * p < 0.05, ** p < 0.01 vs . the SC group. ns, no significant difference. (N–S) Quantitative analysis of protein expression levels of NMDAR, PSD95, SYN, BDNF, c-FOS, and Zif268 in the hippocampus of mice from different groups. Protein expression levels were normalized to Tubulin. Phosphorylated proteins were further normalized to their corresponding total protein levels. Relative gene expression was quantified and normalized to Gapdh. For comparisons among multiple groups, one-way ANOVA was applied. The data are presented as mean ± SD. n = 6. * p < 0.05, ** p < 0.01 vs . the SC group. ns, no significant difference. SC, scramble control, sedentary; SCH, scramble control + HIIT; GKD, GPR81 knockdown, sedentary; GKDH, GPR81 knockdown + HIIT.

Techniques Used: Activation Assay, Expressing, Western Blot, Gene Expression, Control, Knockdown

GPR81-induced modulation of ERK1/2 via AC-RAP1 and PLC-PKC pathways regulates synaptic plasticity-related protein expression in N2a cells. (A,I) Representative Western blotting images. (B–H,J–P) Quantitative analysis of NMDAR, PSD95, SYN, BDNF, Zif268, c-Fos, and ARC protein expression levels. Protein expression levels were normalized to Tubulin. Phosphorylated proteins were further normalized to their corresponding total protein levels. For comparisons among multiple groups, one-way ANOVA was applied. The data are presented as mean ± SD. * p < 0.05 vs . the DMSO group. n = 3. # p < 0.05, ## p < 0.01, ### p < 0.001 vs . the Ga group. ns, no significant difference. Ga, GPR81 agonist; Ei, ERK1/2 inhibitor; Aa, AC agonist; Pi, PLC inhibitor.

Figure Legend Snippet: GPR81-induced modulation of ERK1/2 via AC-RAP1 and PLC-PKC pathways regulates synaptic plasticity-related protein expression in N2a cells. (A,I) Representative Western blotting images. (B–H,J–P) Quantitative analysis of NMDAR, PSD95, SYN, BDNF, Zif268, c-Fos, and ARC protein expression levels. Protein expression levels were normalized to Tubulin. Phosphorylated proteins were further normalized to their corresponding total protein levels. For comparisons among multiple groups, one-way ANOVA was applied. The data are presented as mean ± SD. * p < 0.05 vs . the DMSO group. n = 3. # p < 0.05, ## p < 0.01, ### p < 0.001 vs . the Ga group. ns, no significant difference. Ga, GPR81 agonist; Ei, ERK1/2 inhibitor; Aa, AC agonist; Pi, PLC inhibitor.

Techniques Used: Expressing, Western Blot

Injection:

Article Title: CREB controls cortical circuit plasticity and functional recovery after stroke.
Article Snippet: .. For the immediate early gene study (Fig. 3), 4 weeks after lentiviral injection brains were sliced coronally (40 μm) and incubated with Zif268 (Zinc finger protein 225 or erg-1; 1:750, Cell Signaling, #4153S) or p-CREB (phospho-CREB, 1:800, Cell Signaling, #9198). .. Automatic counting in multiple fluorescent images was performed using ImageJ software, version 1.4 (National Institutes of Health).

Article Title: CREB controls cortical circuit plasticity and functional recovery after stroke
Article Snippet: .. For the immediate early gene study (Fig. ), 4 weeks after lentiviral injection brains were sliced coronally (40 μm) and incubated with Zif268 (Zinc finger protein 225 or erg-1; 1:750, Cell Signaling, #4153S) or p-CREB (phospho-CREB, 1:800, Cell Signaling, #9198). .. Automatic counting in multiple fluorescent images was performed using ImageJ software, version 1.4 (National Institutes of Health).

Incubation:

Article Title: Role of the Plasticity-Associated Transcription Factor Zif268 in the Early Phase of Instrumental Learning
Article Snippet: .. Brain sections were then incubated for 15 min in 0.2% (vol/vol) Triton X-100 in TBS, rinsed 3 times in TBS and blocked in 3% (weight/vol) bovine serum albumin–TBS solution before incubation overnight at 4°C with either rabbit monoclonal antibody directed against Zif268 (1∶1000, Cell Signaling Technology, ref. 15f7) or rabbit polyclonal antibody directed against c-Fos (1∶1000; Santa Cruz Biotechnology: ref. sc-52). ..

Planar Chromatography:

Article Title: HIIT-induced lactate/GPR81 signaling with dual branches converging on ERK1/2 contributes to hippocampal synaptic remodeling and memory improvement
Article Snippet: After a 15-min blocking step (PS108P, Epizyme, Shanghai, China), the samples were incubated with primary antibodies overnight at 4 °C. .. GPR81, PA5-114741, Thermo; AC (55067-1-AP, Proteintech), PKA (4782S, CST), RAP1 (2399S, CST), PKC (SC-80 Santa), PLC (14247S, CST), Raf1 (9422S, CST), MSK1 (3489S, CST), P90RSK (9355S, CST), ERK (4370T, CST), CREB (9197S, CST), c-Fos (2250S, CST), Zif268 (4154S, CST), NMDAR (5704S, CST), SYN (4329S, CST), BDNF (28205-1-AP, Proteintech), ARC (16290-1-AP, Proteintech), PSD95 (3450S, CST) p-Raf1 (9421S, CST), p-ERK (4370T, CST), p-MSK (9595S, CST), p-P90RSK (11989S, CST), p-CREB (9198S, CST), Tubulin (10068-1-AP, Proteintech), HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H + L) (SA00001-2, Proteintech), HRP-conjugated Affinipure Goat Anti-Mouse IgG (H + L) (SA00001-1, Proteintech). ..

PAMPA Assay:

Article Title: Developmental changes in plasticity, synaptic, glia, and connectivity protein levels in rat basolateral amygdala
Article Snippet: .. Primary antibodies against the following proteins were obtained from the indicated suppliers: Arc (1:10000, Synaptic System, cat# 156 003), c-Fos (1:200, Millipore, cat# PC05), Zif268 (1:1000, Cell Signaling Technology, cat# 4153S), pTrkB (Tyr816) (1:1000, Millipore, cat# ABN1381), TrkB (80E3) (1:1000, Cell Signaling Technology, cat# 4603S), pCREB (Ser133) (1:1000, Cell Signaling Technology, cat# 9198), CREB (1:1000, Cell Signaling Technology, cat# 9104), pCaMKIIα (Thr286) (1:1000, Cell Signaling Technology, cat# 3361S), CaMKIIα (1:1000, Millipore, cat# 05-532), pERK1/2 (pp44/42 MAPK) (Thr202/Tyr204) (1:2000, Cell Signaling Technology, cat# 9101S), ERK1/2 (p44/42 MAPK) (1:2000, Cell Signaling Technology, cat# 4695S), GluN2A (1:1000, Millipore, cat# 7-632), GluN2B (1:1000, Cell Signaling, cat# 4212S), GluA1 (1:2000, Millipore, cat# AB1504), GluA2 (1:1000, UC Davis/NIH NeuroMab Facility, cat# 75-002), pAMPA receptor GluA1 (Ser845) (1:1000, Cell Signaling Technology, cat# 8084S), pAMPA receptor GluA1 (Ser831) (1:1000, Abcam, cat# ab109464), PSD95 (1:1000, Cell Signaling Technology, cat# 2507S), synaptophysin (1:1000, Cell Signaling Technology, cat# 5467), GAD65 (1:1000, Millipore, cat# ABN101), GAD67 (1:1000, Millipore, cat# AB5406), Gephyrin (1:1000, Synaptic Systems, cat# 147-111), p-cofilin (Ser3) (1:1000, Abcam, cat# ab12866), cofilin (1:1000, Millipore, cat# AB3842), MAP2 (1:1000, Millipore, cat# MAB3418), mTOR (1:1000, Cell Signaling Technology, cat# 4517S), pmTOR (1:1000, Cell Signaling Technology, cat# 2971S), myelin basic protein (MBP) (1:1000, Millipore, cat# 05-675), Nogo-A (1:1000, Abcam, cat# 62024), Nogo-R (1:1000, Abcam, cat# 26291), myelin-associated glycoprotein (MAG) (1:1000, Cell Signaling Technology, cat# 9043S). .. The membranes were washed in TBS with 0.1% Tween-20 (TBST), and then incubated for 1 h at room temperature with a species-appropriate fluorescently conjugated secondary antibody (goat anti-mouse IRDye 680LT [1:10,000] or goat anti-rabbit IRDye 800CW [1:10,000] from LI-COR Bioscience [Lincoln, NE]).

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